Protein and carbohydrate-lipid metabolism in cows that were kept tethered according to the results of dyspanserisation
Abstract
The productivity of cows is related to the level of metabolism in animals. The intensity of metabolic processes depends on the physiological state of the animals. The aim of this study was to establish the metabolic profile of blood serum in cows according to different physiological groups.
The object of the study was 15 cows of the Ukrainian black and white dairy breed kept in the HDZVA. There were 5 animals of the milking period, 5 in dry period and 5 lactating (6 months after calving).
The animals were examined by general clinical methods (temperature, pulse, and respiratory rate were determined) and blood was taken from the jugular vein for biochemical research. Content of total protein, albumins, globulins, urea, creatinine, total cholesterol, the concentration of glucose and the activity of liver-indicator enzymes - alanine and aspartic transaminases (ALT, ASAT) were determined in blood serum. Blood samples (n = 15) were examined by the following methods: protein content was determined using the biuret reaction, the concentration of albumins and globulins using precipitation with saline solutions, urea – by the method with diacetyl monooxime, creatinine – by the kinetic reaction, cholesterol – by the Jendrashik method, glucose – by glucoseoxidase reaction, activity of transaminases according to Raitman-Frenkel. Numerical data were statistically processed using standard «Microsoft Excel» programs, using Student’s t-criteria.
In cows after parturition the level of total protein in blood serum was lower by 7.9% compared with the norm. The concentration of albumins was reduced in lactating animals after parturition and the period of six months after calving by 19.5 % and 20.9 %, respectively. This indicates a decrease in the protein-forming function of the liver.
Concentration of cholesterol in blood serum of lactating animals was lower by 34.9%, which may indicate both a lack of energy resources in the diet of animals and a malfunction of the thyroid gland.
We recommend to investigate the nutritional value of all feeds used on the farm for feeding cattle, in order to exclude imbalances in the diet in terms of content and balance of nutrients.
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References
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