The parameters’ determination of acute toxicity of food additive «Biostim 40» for rats
Abstract
Domestic animals have a positive influence on economic and physical human health because the contact with them increases physical activity and decreases arterial pressure, the risk of hypertensive people to have cardiovascular diseases, stress rate, frequency of depressions and increases the level of self-estimation as well as social activity of children and teens.
Nowadays numerous commercial pet foods for domestic animals are developed with exaggeration of minimal recommendations that can cause eating disorders, metabolic disorders and animals’ obesity, thus, to ensure the protection for animals it is important to pay attention to pharmacological and toxicological analyses of food additives presenting necessary recommendations of their usage.
To determine the acute toxicity of high-energetic protein, vitamin and mineral food additive “Biostim 40” there has been carried out a research on grown-up males of white rats with a mass in 200-230 g. Animals have been divided into 7 groups of 8 rats in every (n=56). Food additive has been fed individually to every rat in doses of 2000, 4000, 6000, 10000, 20000 and 30000 mg/kg per body mass. There have been implemented observations of rats’ general state, behavior, their orientation, reflexes of hearing and vision, eating and drinking, state of their fur and visible mucous membranes, breathing and excrements – all those factors have shown that even the highest doses of “Biostim 40” (30000 mg/kg per body mass), being inserted internally through the abdomen, have not caused any clinical cases of poisoning, rats’ deaths and, in accordance with international classification of chemical substances, “Biostim 40” may be enlisted to the 4th level of toxicity – the substance of low toxicity.
Analyzing the dynamic of rats’ changing their body mass within 2 weeks it is possible to point out that one-time insertion of high doses of “Biostim 40” has assisted to increase rats’ body mass in 14-33% (р≤0.05), with the help of what we can say that this food additive is effective and safe stimulator of height and development of animals.
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References
Ayaso, R., Ghattas, H., Abiad, M., & Obeid, O. (2014). Meal Pattern of Male Rats Maintained on Amino Acid Supplemented Diets: The Effect of Tryptophan, Lysine, Arginine, Proline and Threonine. Nutrients, 6(7), 2509-2522. doi:10.3390/nu6072509.
Beski, S. S. M., Swick, R. A., & Iji, P. A. (2015). Specialized protein products in broiler chicken nutrition: A review. Anim Nutr. 1(2): 47-53. doi:10.1016/j.aninu.2015.05.005.
Biourge, V. C., Fontaine, J., & Vroom, M. W. (2004). Diagnosis of Adverse Reactions to Food in Dogs: Efficacy of a Soy-Isolate Hydrolyzate-Based Diet. The Journal of Nutrition, 134(8), 2062-2064. doi.org/10.1093/jn/134.8.2062S.
Bokova, T. I. (2008). Ispol'zovaniye biologicheski aktivnykh dobavok v ratsione sel'skokhozyaystvennykh zhivotnykh. Kormleniye sel'skokhozyaystvennykh zhivotnykh i kormoproizvodstvo, (9), 61-62 (in Russian).
Elinskaya, O. L. (2011). Gigiyenicheskiye aspekty primeneniya pishchevykh dobavok (spravochno-metodicheskoye posobiye). Tashkent (in Russian).
Kotsyumbas, I. YA. et al. (2006). Doklinichni doslidzhennya veterynarnykh likarsʹkykh zasobiv. Lʹviv : Tryada plyus. 63-85, 136-149 (in Ukrainian).
Lemon, P. W. (1995). Do athletes need more dietary protein and amino acids? International journal of sport nutrition, 5(s1), 39–561. doi:10.1123/ijsn.5.s1.s39.
Lemyaseva, S. V. (2013). Ispol'zovaniye stimulyatorov rosta sel'skokhozyaystvennykh zhivotnykh i bezopasnost' produktov zhivotnovodstva. Problemy veterinarnoy sanitarii, gigiyeny i ekologii, 1(9), 97-101 (in Russian).
Malinin, O. A., Khmel'nitskiy, G. A., & Kutsan, A. T. (2002). Veterinarnaya toksikologiya : ucheb. posobiye. Korsun-Shevchenkovskyi: ChP Maidachenko, 464 (in Russian).
Mosoni, L., Gatineau, E., Gatellier, Ph., Migné, C., Savary-Auzeloux, I., Rémond, D., Rocher, E., & Dardevet, D. (2014). High Whey Protein Intake Delayed the Loss of Lean Body Mass in Healthy Old Rats, whereas Protein Type and Polyphenol/Antioxidant Supplementation Had No Effects. PLoS One, 9(9), e1090-98. doi:10.1371/journal.pone.0109098.
Pressman, P., Clemens, R. A., & Hayes, A. W. (2017). Bioavailability of micronutrients obtained from supplements and food: A survey and case study of the polyphenols. PLoS One, 1, 1-7. doi.org/10.1177/2397847317696366.
Prozorovskiy, V. B. (1962). Ispol'zovaniye metoda naimen'shikh kvadratov dlya probit-analiza krivykh letal'nosti. Farmakologiya i toksikologiya, 1-3, 115-120 (in Russian).
Rotimi1, O. A., Rotimi1, S. O., Oluwafemi, F., Ademuyiwa, O., & Balogun, E. A. (2018). Oxidative Stress in Extrahepatic Tissues of Rats Co-Exposed to Aflatoxin B1 and Low Protein Diet. Toxicological Research, 34, 211-220. doi.org/10.5487/TR.2018.34.3.211.
Rubinskiy, I. A., & Petrova, O. G. (2012). Immunnyye stimulyatory v veterinarii (teoreticheskiye i eksperimental'nyye osnovy) Retrieved from http://www.kniga.com/books/preview_txt.asp?sku=ebooks324510 (in Russian).
Sherkuziyeva, G. F., Sharipova, S. A., & Khaydarov, SH. M. (2016). Rezul'taty izucheniya ostroy toksichnosti «Laktonorm-H (K kaliy)». Molodoy uchenyy, 26, 221-223 (in Russian).
Sobakar, A. V., Zhukova, I. O., & Lonhus, N. I. (2016). Perspektyva zastosuvannya preparatu «Biostym 40» dlya sobak ta kishok. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 18(1), 154-157. Retrieved із https://nvlvet.com.ua/index.php/journal/article/view/63 (in Ukrainian).
Swanson, K. S., Carter, R. A., Yount, T. P., Aretz, J., & Buff, P. R. (2013). Nutritional Sustainability of Pet Foods. Advances in Nutrition, 4(2), 141-150. doi:10.3945/an.112.003335.
Tarnopolsky, M. A., Atkinson, S. A., MacDougall, J. D., Chesley, A., Phillips, S., & Schwarcz, H. P. (1992). Evaluation of protein requirements for trained strength athletes. Journal of Applied Physiology, 73(5), 1986–1995. doi:10.1152/jappl.1992.73.5.1986.
Wu Guoyao. (2010). Functional Amino Acids in Growth, Reproduction, and Health. Adv Nutr. Nov, 1(1), 31-37. doi:10.3945/an.110.1008.
Yang, Ch., Chowdhury, M. A. K., Hou, Y., & Gong, J. (2015). Phytogenic Compounds as Alternatives to In-Feed Antibiotics: Potentials and Challenges in Application. Pathogens, 4(1), 137–156. doi.org/10.3390/pathogens4010137.
Zapadnyuk, I. P., Zapadnyuk, V. I., & Zakhariya, Ye. A (1974). Laboratornyye zhivotnyye. Razvedeniye, soderzhaniye, ispol'zovaniye v eksperimente, 304 (in Russian).
Zdarilova, A., Vrublova, E., Vostalova, J., Klejdus, B., Stejskal, D., Proskova, J., … Simanek, V. (2008). Natural feed additive of Macleaya cordata: Safety assessment in rats a 90-day feeding experiment. Food and Chemical Toxicology, 46(12), 3721-6. doi: 10.1016/j.fct.2008.09.054.
Zinchenko, D. A. (2014). Vliyaniye tkanevykh biostimulyatorov na organizm zhivotnykh. Mezhdunarodnyy zhurnal prikladnykh i fundamental'nykh issledovaniy, (3-1), 124-125 (in Russian).
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