Determination of acute toxicity of «Fipren» preparation

Abstract

Biological protection of farms is impossible without an effective and timely implementation of a set of veterinary and sanitary measures, in particular pest control. Systematic rotation of ectocides is a predisposing factor for successful processing. Therefore, the development of domestic insectoacaricid preparation is topical. Scientists of the "Brovafarma" NPF developed an insecticacicid preparation "Fipren", which contains in its composition a composition of synergistically active components fipronil and (S)-methoprene. Active substances of the preparation are effective provided insects and arthropods have resistance to pyrethroids, carbamates. The development of a new preparation requires a thorough pharmaco-toxicological assessment, in particular the determination of acute toxicity of the preparation in laboratory animals. Materials and methods. Determination of acute toxicity parameters of the study preparation "Fipren" was carried out on 40 healthy mongrel white mice. The body weight of the animals before the start of the experiment was 18-20 g. The test agent was administered to white mice intragastrically by means of a probe in the following doses: 200, 500, 800, 1100, 1400, 1700, 2000, 2300, 2600, 2900 mg/kg in the first stage experience. For the basic experiment, five groups of analog mice were formed (n=30). The test agent was administered to white mice in the following doses: 500, 1000, 1500, 2000 and 2500 mg/kg. The animals of the experiment were monitored continuously during the first 24 hours after the administration of the preparation, and during the following 13 days, the dynamics of changes in their clinical state were noted. The results of research. As a result of the approximate stage of the study, the absence of death of the experimental animals was determined in the preparation of the "Fipren" preparation in doses of 200, 500, 800, 1100, 1400 mg/kg during the whole observation period. Administration of the preparation at a dose of 1700, 2000, 2300, 2600, 2900 mg/kg caused the death of experimental mice at different time intervals after administration and with different signs. The obtained rates of animal death were taken into account during the main stage of acute experience. When it was administered, the preparation was administered in the following doses: 500, 1000, 1500, 2000 and 2500 mg/kg. Administration of the drug at a dose of 500 mg/kg did not lead to the death of experimental animals. The introduction of the preparation "Fipren" in a dose of 2500 mg / kg resulted in the death of 100% of white mice. Conclusions. At intragastric administration, when calculating by G. Kerber's method for white mice, DL50 of the preparation was 1250 mg/kg. The preparation «Fipren» in accordance with GOST 12.1.007-76 refers to the fourth class of toxicity - low-toxic substances.