Microscopic structure of some digestive visces of European Roes in esherichiosis

Abstract

We examined the corpses of two specimens of European goats, both - females, the age of about 3-4 months, caught in the wild for transportation to the hunting industry; the animals died with signs of dehydration and diarrhea the second day after catching. At the autopsy, changes were observed, typical for gastrointestinal infections; bacteriological studies confirmed the presence of pathogenic strains of E. coli. The pieces of material for histological examination were selected from the small and large intestines, liver, pancreas. The selected material was fixed in a 10% aqueous solution of formalin, poured into paraffin, microscopic cutters were made in a thickness of 10 μm, stained with hematoxylinum of Karachi and eosin, studied under a light microscope and made microphotographs. In the small intestine, the destruction of the villi was observed. Intestinal glands are enlarged, filled with mucus content. In the lumen of the gut contained a mucous substance containing desquamated cells of the intestinal epithelium. In the fibrous connective tissue of its own plate and submucosal basis, collagen fibers are loose, flaky, poorly stained, indicating the development of edema. In addition, lymphocytic infiltration of connective tissue was observed, especially between the villi. Similar changes were observed in the colon, most pronounced they were in the rim. In addition, there was a pronounced hyperemia of vessels of its own plate and submucosal basis, vessels enlarged, filled with blood. In the liver, blood vessels, both in the lobes, and between the lobar, enlarged, overflowing with blood. In the thickness of the lobules were spotted hemorrhages. A large number of hepatocytes in the state of granular dystrophy – these cells had an inhomogeneously colored cytoplasm with a characteristic "foamy" appearance, their nuclei were poorly stained. Separate hepatocytes were in the state of fatty cell decompositional dystrophy - their cytoplasm had a rather transparent appearance, the nuclei retained their central position in the cell. In the pancreas, marked hyperemia of the vessels, the acinus of the gland was filled with translucent secretion; some cells of the exocrine part were destroyed. The detected microscopic changes in digestive organs for colibacteriosis in young European roes differ significantly from those in calves and lambs; Judging by the revealed changes, the disease was subacute with the tendency of transition to chronic; Intestines were shown signs of acute catarrhal inflammation, in the liver – acute venous hyperemia and granular and fatty degeneration of hepatocytes, in the pancreas - the development of subacute pancreatitis. Further researches need to establish the features of microscopic changes in other organs and tissues.