Distribution and features of the clinical manifestation of rhinotracheitis of cats in Kharkiv
Abstract
Feline respiratory infectious diseases have spread to many countries around the world.
The isolation of a specific pathogen and its identification are of great importance. It is also important for the use of specific agents for the treatment and prevention of these diseases.
In 2019-2020 the studies at the private veterinary clinic «Aibolit» in Kharkiv were conducted in the conditions of private breeding cattery for cats in the city and using the equipment of the educational - scientific laboratory of molecular - genetic research methods at KHZVA.
Materials for research were cats of different breeds and sex-age groups with clinical signs of respiratory diseases, as well as biomaterials from them (blood and nasal discharge), which were studied both by virological methods and PCR.
The nosological profile of respiratory infectious diseases of cats in Kharkiv is formed by 3 nosological units. In 24% of cases, the clinical manifestations of rhinotracheitis in cats were caused by the association of two or three pathogens (calicivirus, herpesvirus and chlamydia). The herpes viruses accounted for 33.1% of the total samples of cats’ biomaterials that were examined.
From animals with infection using PCR herpes virus was isolated in 83.8% of cases. In 55 cases (42.6%) this virus was the only pathogen that was detected.
The analysis of the disease of cats taking into account their age showed that rhinotracheitis was more often affected by young cats at the age of 1.5-3.5 months to 5.5 years and older. Cats that were kept individually and in groups were ill.
Cats of certain breeds were sick. Cases of the disease were recorded more often in spring and autumn.
The disease proceeded in respiratory, conjunctival and less often in genital forms.
The main pathway to isolate the rhinotracheitis pathogen into the external environment was respiratory, with outflows from the nasal cavity of animals, where they were detected in an amount of 2.03 ± 0.12 lg TCD 50 / ml - 2.05 ± 0.16 lg TCD 50 / ml. (by age group) even with latent infection. The pathogen was isolated in 38,4% of cats with clinical manifestations of the disease using PCR. In 23.8% of cases the herpes virus was isolated as the only pathogen.
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References
Bannasch, M. J., & Foley, J. E. (2005). Epidemiologic evaluation of multiple respiratory pathogens in cats in animal shelters. J Feline Med Surg., 7 (2), 109-19. DOI: 10.1016/j.jfms.2004.07.004
Bergmann, M., Ballin, A., Schulz, B., Dörfelt, R., & Hartmann, K. (2019). [Treatment of acute viral feline upper respiratory tract infections]. Tierarztl Prax Ausg K Kleintiere Heimtiere, 47(2), 98-109. DOI: 10.1055/a-0870-0801
Chen, T., Zhou, X., Qi, Y., Mi, L., Sun, X., Zhang, S. … Hu, R. (2019).Feline herpesvirus vectored-rabies vaccine in cats: A dualprotection. Vaccine, 10, 37 (16), 2224 - 2231. DOI: 10.1016 /j.vaccine.2019.03.008
Contreras, E. T., Olea-Popelka, F., Wheat, W., Dow, S., Hawley, J., & Lappin, M. R. (2019). Evaluation of liposome toll-like receptor ligand complexes for non-specific mucosal immunoprotection from feline herpesvirus-1 infection. Vet Intern Med., 33(2), 831-837. DOI: 10.1111 / jvim.15427
Dinnage, J. D., Scarlett, J. M., & Richards, J. R. (2009). Descriptive epidemiology of feline upper respiratory tract disease in an animal shelter. J Feline Med Surg.,11, 816‐825. DOI: 10.1016/j.jfms.2009.03.001
Gould, D. (2011). Feline herpesvirus-1: ocular manifestations, diagnosis and treatment options. J Feline Med Surg., 13(5), 333-346. DOI: 10.1016/j.jfms.2011.03.010
Helps, C. R., Lait, P., Damhuis, A., Björnehammar, U., Bolta, D., Brovida, C., & Graat, E. A. (2005). Factors associated with upper respiratory tract disease caused by feline herpesvirus, feline calicivirus, Chlamydophilafelis and Bordetella bronchiseptica in cats: experience from 218 European catteries. Vet Rec., 21, 156(21), 669-73. DOI: 10.1136/vr.156.21.669
Lappin, M. R., Blondeau, J., Boothe, D., Breitschwerdt, E. B., Guardabassi, L., Lloyd, D. H. … Weese, J. S. (2017). Antimicrobial use Guidelines for Treatment of Respiratory Tract Disease in Dogs and Cats: Antimicrobial Guidelines Working Group of the International Society for Companion Animal Infectious Diseases.J Vet Intern Med., 31(2), 279-294. DOI: 10.1111/jvim.14627
Legendre, A. M., Kuritz, T., Heidel, R. E., & Baylor, V. M. (2017). Polyprenyl Immunostimulant in Feline Rhinotracheitis: Randomized Placebo-Controlled Experimental and Field Safety Studies. Front Vet Sci., 27(4), 24. DOI: 10.3389/fvets.2017.00024
Litster, A., Wu, C. C., & Leutenegger, C. M. (2015). Detection of feline upper respiratory tract disease pathogens using a commercially available real-time PCR test. Vet J., 206(2), 149-53. DOI: 10.1016/j.tvjl.2015.08.001
Malik, R., Lessels, N. S., Webb, S., Meek, M., Graham, P.G., Vitale, C., Norris J.M., & Power, H. (2009). Treatment of feline herpesvirus-1 associated disease in cats with famciclovir and related drugs. J Feline Med Surg., 11(1), 40-8. DOI: 10.1016/j.jfms.2008.11.012
Reagan, K. L., Hawley, J. R., & Lappin, M. R. (2014). Concurrent administration of an intranasal vaccine containing feline herpesvirus-1 (FHV-1) with a parenteral vaccine containing FHV-1 is superior to parenteral vaccination alone in an acute FHV-1 challenge model. Vet J., 201(2), 202-206. DOI: 10.1016 / j.tvjl.2014.05.003
Spiri, A. M., Meli, M. L., Riond, B., Herbert, I., Hosie, M. J., & Hofmann-Lehmann, R. (2019). Environmental Contamination and Hygienic Measures After Feline Calicivirus Field Strain Infections of Cats in a Research Facility. Viruses., 17,11(10). DOI: 10.3390/v11100958
Summers, S. C., Ruch-Gallie, R., Hawley, J. R., & Lappin, M. R. (2017). Effect of modified live or inactivated feline herpesvirus-1 parenteral vaccines on clinical and laboratory findings following viral challenge. J Feline Med Surg., 19(8), 824-830. DOI: 10.1177/1098612X16659333
Sykes, J. E., Allen, J. L., Studdert, V. P., & Browning, G. F. (2001). Detection of feline calicivirus, feline herpesvirus 1 and Chlamydia psittaci mucosal swabs by multiplex RT-PCR/PCR.Vet Microbiol., 26, 81(2), 95-108. DOI: 10.1016 /s0378-1135 (01) 00340-6
Tan, Y., Dong, G., Niu, J., Guo, Y., Yi, S., Sun, M., Wang, K., & Hu, G. (2019). Development of an indirect ELISA based on glycoprotein B gene for detecting of Feline herpesvirus type 1. PolJVetSci., 22(3), 631-633. DOI: 10.24425/pjvs.2019.129971
Tan, Y., Dong, G., Xu, H., Niu, J., Lu, W., Wang, K., & Hu, G. (2020). Development of a cross-priming isothermal amplification assay based on the glycoprotein B gene for instant and rapid detection of feline herpesvirus type 1. ArchVirol., 165(3), 743-747. DOI: 10.1007/s00705-020-04526-5
Van Brussel, K., Carrai, M., Lin, C., Kelman, M., Setyo, L., Aberdein, D., & Barrs, V. R. (2019). Distinct Lineages of Feline Parvovirus Associated with Epizootic Outbreaks in Australia, New Zealand and the United Arab Emirates. Viruses., 13, 11(12). DOI: 10.3390/v11121155
Wang, J., Liu, L., Wang, J., Sun, X., & Yuan, W. (2017). Recombinase Polymerase Amplification Assay-A Simple, Fast and Cost-Effective Alternative to Real Time PCR for Specific Detection of Feline Herpesvirus-1.PLoS One, 12(1), e0166903. DOI:10.1371 / journal.pone.0166903
Wheat, W., Chow, L., Coy, J., Contreras, E., Lappin, M., & Dow, S. (2019). Activation of upper respiratory tract mucosal innate immune responses in cats by liposomal toll-like receptor ligand complexes delivered topically. J Vet Intern Med., 33(2), 838-845. DOI: 10.1111/2Fjvim.15426
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